However, cytometers such as BD Fortessa use Hierarchical gating. The old school Cell Quest and Cell Quest Pro software of a BD FACSCalibur is an example of a cytometer that used Boolean Gating. This depends on the cytometer you use and the question you are asking. When selecting the cells of interest from your sample, you might choose hierarchical gating or Boolean gating. There are two main ways of gating your cells: hierarchical and Boolean gating. As cytometrists, we gate our cells to exclude unwanted bits and to focus on the sub-populations that we are interested in studying. This includes debris and even bits in your buffers. A flow cytometer collects the events you are interested in, and also ‘sees’ every event that goes through.
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